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In Vitro

Boost oncology drug discovery with XenoBase®, featuring the largest cell line selection and exclusive 3D organoid models. Benefit from OrganoidXplore™ and OmniScreen™ for rapid, in-depth analysis.

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In Vivo

Enhance drug development with our validated in vivo models, in vitro/ex vivo assays, and in silico modeling. Tailored solutions to optimize your candidates.

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Tissue

Experience ISO-certified biobanking quality. Access top biospecimens from a global clinical network, annotated by experts for precise research.

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Biomarkers and Bioanalysis

Leverage our global labs and 150+ scientists for fast, tailored project execution. Benefit from our expertise, cutting-edge tech, and validated workflows for reliable data outcomes.

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Data Science and Bioinformatics

Harness your data and discover biomarkers with our top bioinformatics expertise. Maximize data value and gain critical insights to accelerate drug discovery and elevate projects.

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KRAS

Accelerate innovative cancer treatments with our advanced models and precise drug screening for KRAS mutations, efficiently turning insights into clinical breakthroughs.

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EGFR

Advance translational pharmacology with our diverse pre-clinical models, robust assays, and data science-driven biomarker analysis, multi-omics, and spatial biology.

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Drug Resistance

Our suite integrates preclinical solutions, bioanalytical read-outs, and multi-omics to uncover drug resistance markers and expedite discovery with our unique four-step strategy.

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Patient Tissue

Enhance treatments with our human tumor and mouse models, including xenografts and organoids, for accurate cancer biology representation.

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Target to Lead Selection for ADCs and Biologics

Journey through the drug discovery pipeline from target discovery to in vivo pharmacology. Take advantage of the Largest biobank of patient-derived models, Model development capabilities, Cell-based assays for Screening ADCs, and advanced downstream analysis.

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Bioinformatics

Apply the most appropriate in silico framework to your pharmacology data or historical datasets to elevate your study design and analysis, and to improve your chances of clinical success.

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Biomarker Analysis

Integrate advanced statistics into your drug development projects to gain significant biological insight into your therapeutic candidate, with our expert team of bioinformaticians.

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CRISPR/Cas9

Accelerate your discoveries with our reliable CRISPR solutions. Our global CRISPR licenses cover an integrated drug discovery platform for in vitro and in vivo efficacy studies.

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Genomics

Rely on our experienced genomics services to deliver high quality, interpretable results using highly sensitive PCR-based, real-time PCR, and NGS technologies and advanced data analytics.

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In Vitro High Content Imaging

Gain more insights into tumor growth and disease progression by leveraging our 2D and 3D fluorescence optical imaging.

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Mass Spectrometry-based Proteomics

Next-generation ion mobility mass spectrometry (MS)-based proteomics services available globally to help meet your study needs.

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Ex Vivo Patient Tissue

Gain better insight into the phenotypic response of your therapeutic candidate in organoids and ex vivo patient tissue.

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Spatial Multi-Omics Analysis

Certified CRO services with NanoString GeoMx Digital Spatial Profiling.

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Biomarker Discovery

De-risk your drug development with early identification of candidate biomarkers and utilize our biomarker discovery services to optimize clinical trial design.

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DMPK Services

Rapidly evaluate your molecule’s pharmaceutical and safety properties with our in vivo drug metabolism and pharmacokinetic (DMPK) services to select the most robust drug formulations.

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Efficacy Testing

Explore how the novel HuGEMM™ and HuCELL™ platforms can assess the efficacy of your molecule and accelerate your immuno-oncology drug discovery programs.

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Laboratory Services

Employ cutting-edge multi-omics methods to obtain accurate and comprehensive data for optimal data-based decisions.

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Pharmacology & Bioanalytical Services

Leverage our suite of structural biology services including, recombinant protein expression and protein crystallography, and target validation services including RNAi.

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Screens

Find the most appropriate screen to accelerate your drug development: discover in vivo screens with MuScreen™ and in vitro cell line screening with OmniScreen™.

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Toxicology

Carry out safety pharmacology studies as standalone assessments or embedded within our overall toxicological profiling to assess cardiovascular, metabolic and renal/urinary systems.

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Preclinical Consulting Services

Learn more about how our consulting services can help to support your journey to the clinic.

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Our Company

Global CRO in California, USA offering preclinical and translational oncology platforms with high-quality in vivo, in vitro, and ex vivo models.

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Learn more about the impact we make through our scientific talent, high-quality standards, and innovation.

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Our Responsibility

We build a sustainable future by supporting employee growth, fostering leadership, and exceeding customer needs. Our values focus on innovation, social responsibility, and community well-being.

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Meet Our Leadership Team

We build a sustainable future by fostering leadership, employee growth, and exceeding customer needs with innovation and social responsibility.

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Our Scientific Advisory Board of experts shapes our strategy and ensures top scientific standards in research and development.

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Oncology Cell Lines for Research Applications

cell microscopy image

cell microscopy imageExplore the main types of cancer cell line available and some of their common utilities.

Oncology Cell Lines

One of the backbones of cancer research is cell-based assays and studies. Following many years of scientific development there’s now a wide range of cell line types available, which are reviewed in this post.

Primary Cells

Primary cells are cancer cells isolated directly from human or animal tissue. As they haven’t been immortalized, they have a finite lifespan, but also lack modifications (other than those which occur during culture).

Unlike cell lines, primary cells are from multiple donors which can prove useful for early drug testing. They also allow you to study donors rather than just cells, which can be beneficial in personalized medicine studies.

Cancer Cell Lines

One of the most common tools used in cancer research is immortalized human cancer cell lines, such as the collections available from the ATCC® and ECACC. They are easier to use and more robust than primary cells, with large numbers available – the ATCC alone maintains almost 4,000 cell lines. Cell lines are often highly proliferative and easier in general to culture, and to transfect, then primary cell alternatives.

While human cancer cell lines have helped numerous scientific advances and have many cell-based applications they are not without drawbacks. Misidentification and contamination can be a major issue affecting cell line research. Adaptation to growth on plastic also induces changes within cell lines, which results in alteration in genotype and phenotype from the original disease.

Engineered Cell Lines

With the advancement of scientific techniques, a range of engineered cell lines are now available for a variety of assay types, including:

  • Knock-in cell lines – created using rAAV or CRISPR, for example, knock-in cell lines recapitulate disease relevant mutations allowing a clear understanding of gene or mutation phenotype function

  • Knockout cell lines – a gene of interest is disabled, by technologies such as CRISPR, with complete loss of gene function occurring allowing function assessment assays

  • Recombinant overexpression cell lines – created with lentiviral vectors, often with reporter molecules (fluorescent or luciferase) for a wide range of research applications including functional assays and/or screening of inhibitors or activators

Cell Line Vendors

Some of the main cell line repositories are discussed above - the ATCC® (extensively used) and ECACC.

Some other popular vendors for modified cell lines include:

  • BPS Bioscience, which supplies many luciferase reporter cells lines, and also has a cell line rental service for select cell lines

  • Abeomics, who supply many stable reporter cell lines including target promoters or upstream response elements for TLRs/RLRs, GPCR, RORs, IL-17A, IL-8, IL-6, p53, Nrf2, Wnt, STAT-1, -3, -4 and -5, NFAT, NF-kB, and more

  • GenScript, providing many immune checkpoint stable cell lines for immunotherapy drug discovery programs

  • Horizon, with ready made cell lines, cell line engineering and KI/KO cell lines available


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